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News November 2011 /4 Plant Genomic DNA Extraction Mini Kit Genomic DNA Mini Kit provides a fast and simple method to isolate total DNA (genomic DNA, mitochondrial and chloroplast) from plant tissue and cells. In the process, sample is distrusted by grinding in liquid nitrogen and lysis buffer incubation. The Lysate is treated with RNase A to degrade RNA and filtrated by filter column to remove cell debris and salt precipitations. In the presence of binding buffer with chaotropic salt, the genomic DNA in the lysate binds to glass fiber matrix in the spin column. The contain are washed with an ethanol contained wash buffer and finally, the purified genomic DNA is eluted by low salt elution buffer or water. The protocol does not require DNA phenol extraction and alcohol precipitation. DNA is ready for PCR, real-time PCR, Southern blotting and RFLP.
Available also as Maxi Kits. Further available Extraction Kits for DNA Fragments, Plasmid DNA, Blood- and Tissue genomic DNA.
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